Munc-18-1 Regulates the Initial Release Rate of Exocytosis

Munc-18-2 regulates exocytosis of H(+)-ATPase in rat inner medullary collecting duct cells.

Exocytic insertion of H(+)-ATPase into the apical membrane of inner medullary collecting duct (IMCD) cells is dependent on a soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein target receptor (SNARE) complex. In this study we determined the role of Munc-18 in regulation of IMCD cell exocytosis of H(+)-ATPase. We compared the effect of acute cell acidification (the stimulus for I...

Munc 18 domain - 3 a in exocytosis 1 1 The domain - 3 a of Munc 18 - 1 plays a crucial role at the priming stage of exocytosis 2 3 4 5

1 1 The domain-3a of Munc18-1 plays a crucial role at the priming stage of exocytosis 2 3 4 5 Gayoung Anna Han, Na-Ryum Bin, Soo-Young Ann Kang, Liping Han and Shuzo Sugita 6 7 Address: Division of Fundamental Neurobiology, University Health Network, 399 Bathurst Street, 8 Toronto, Ontario, M5T 2S8, Canada; Departments of Physiology, Faculty of Medicine, University of 9 Toronto, Ontario, M5S 1A...

Munc 18 - 1 - regulated stage - wise SNARE assembly underlying 4 synaptic exocytosis

Munc 18 - 1 regulates the cortical F - actin network in chromaffin cells

The cortical F-actin network in adrenal chromaffin cells was proposed to be both a barrier and a facilitator for secretory vesicle trafficking and fusion and is reported to be increased in secretion-deficient cells lacking Munc18-1. To investigate this effect of Munc18-1 deficiency on the actin network, we expressed Munc18-1, Munc18-1 orthologues and Munc18-1 mutants in munc18-1 null CCs and an...

The pancreatic ß-cell protein Granuphilin binds Rab3 and Munc-18 and controls exocytosis

Granuphilin/Slp-4 is a member of the Synaptotagmin-like protein family expressed in pancreatic ß-cells and in pituitary gland. We show by confocal microscopy that both Granuphilin-a and -b colocalize with insulin-containing secretory granules positioned at the periphery of pancreatic ß-cells. Overexpression of Granuphilins in insulinsecreting cell lines caused a profound inhibition of stimulus-...